Part:BBa_K4773008:Design
RBS-ptb
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 338
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The ptb gene does not have any EcoRI and PstI restriction sites, which are commonly used for cloning into standard plasmid vectors. Therefore, alternative restriction enzymes or methods may be needed to insert the ptb gene into the desired vector. There is a KpnI site behind the ptb gene, which can be used for downstream manipulation of the part.
Source
This part is a gene encoding the ptb protein from Mycobacterium tuberculosis, which can degrade butyrate to acetyl-CoA and propionyl-CoA in the presence of NAD+ and CoA. This part can be used to construct biocatalysts for butyrate utilization or biofuel production. The part does not contain any EcoRI and PstI restriction sites, but there is a KpnI site behind the ptb gene to facilitate cloning into standard plasmid vectors.